Ierce Firefly Luciferase Glow Assay Kit

Description: Minimum order quantity: 1 unit of 150TST

Description: The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features:• Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity.• Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time• High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications• Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.

Description: The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features:• Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity.• Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time• High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications• Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.

Description: The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features:• Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity.• Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time• High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications• Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.

Description: Recombinant K562 cells constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter.

Description: Recombinant Raji cells constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter.

Description: This enzyme is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis) that provides the reliability and dependability needed for performing research or producing kits using bioluminescence reagents to detect ATP or luciferin substrates.

Description: Recombinant NALM6 cells constitutively expressing the firefly (Photinus pyralis) luciferase reporter gene under the control of a CMV promoter.

Description: Minimum order quantity: 1 unit of 15ML

Description: Firefly Luciferase, also known as Luciferin 4-monooxygenase, Uniprot No. Q27757, a.a. 1-545(full length) with C-terminal His-tag, expressed in an E. coli expression system. MW=61 kDa.

Description: Firefly Luciferase, also known as Luciferin 4-monooxygenase, Uniprot No. Q27757, a.a. 1-545(full length) with C-terminal His-tag, expressed in an E. coli expression system. MW=61 kDa.

Description: Firefly Luciferase, also known as Luciferin 4-monooxygenase, Uniprot No. Q27757, a.a. 1-545(full length) with C-terminal His-tag, expressed in an E. coli expression system. MW=61 kDa.

Description: Recombinant Jurkat cells constitutively expressing the firefly (Photinus pyralis) luciferase reporter  gene under the control of a CMV promoter.

Description: The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. This virus constitutively express firefly luciferase under a CMV promoter.

Description: The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses constitutively express firefly luciferase under a CMV promoter.

Description: Luciferase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (1-311 a.a) and having a molecular mass of 38.5kDa.; Luciferase is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Description: Premade recombinant adenovirus containing the luciferase gene

Description: Recombinant stable NK-92 cell line constitutively expressing firefly (Photinus pyralis) luciferase under the control of a CMV promoter.

Description: Recombinant THP-1 cells constitutively expressing the firefly (Photinus pyralis) luciferase gene under the control of a CMV promoter.