Effect of HBO therapy on adipose-derived stem cells, fibroblasts and co-cultures: In vitro study of oxidative stress, angiogenic potential and production of pro-inflammatory growth factors in co-cultures1
Background: A key moderator of wound healing is oxygen. The healing of the wounds is a dynamic and carefully orchestrated process involving blood cells, cytokines, parenchymate cells (ie fibroblasts and mesenchymate stem cells) and an extracellular matrix reorganization. Stem cells derived from human adiposus as well as human fibroblasts produce soluble factors, have various effects on inflammation and anti-inflammation response and are involved in wound healing processes. Oxygen therapyHyperbare is an effective treatment for ischemic disorders such as chronic infections or chronic wounds. In vitro effects of hyperbaric oxygen therapy on human cells have been presented in many studies, with the exception of stem cells derived from human adiposity and fibroblasts.
Objective: The objective of this study was to study the effects of hyperbaric oxygen therapy on mono-cultures stem cells derived from human adiposis and fibroblasts.
Methods: Mono- and co-cultures of human adiposse derived stem cells and fibroblasts have been established. These cultures were exposed to hyperbaric oxygen every 24 hours for five consecutive days. The measurement experiments were carried out on the first, third and fifth day. The therapy effects on the expression of VEGF, it and the reactive oxygen species have been studied.
Results: After exposure to hyperbaric oxygen, cell culture showed a significant increase in VEGF expression after 3 and 5 days. All crops have shown a significantly reduced formation of reactive oxygen species in experiments. IL-6 expression decreased during experience in mono-cultures stem cells derived from human adiposis and co-cultures. On the other hand, mono-cultures of human skin fibroblasts showed a considerably increased global expression of IL-6.
Conclusions: Hyperbaric oxygen therapy leads to immunodulation and pro-annihilated effects in an environment resembling a wound of adipose derived stem cells and fibroblasts.
Fibroblast 2 growth factor accelerates epithelial-mesenchymatory transition in keratinocytes during the wound healing process
In the process of healing of the wound, the morphology of keratinocytes on the edge of the wound temporarily changes to a morphology of the spindle, which is thought on which will occur due to a messenchymatory epithelial transition (EMT). Fibroblast growth factor (FGF) 2, also known as the basic FGF, capable of accelerating the closure of the wound by activating vascular endothelial cells and fibroblasts.
We examined the effects of the FGF2 on the morphology of keratinocyte and the EMT in the injured skin. The histological examination of the murine wounds treated with FGF2 revealed that the edge keratinocytes of the wound formed a thickened and multilayer epithelium. In addition, we detected edge keratinocytes from the wound individually migrating to the center of the wound. These migratory keratinocytes have not only exposed a morphology of the spindle, but also a regulated and regulated Viménanian expression, characteristic of the EMT.
In the wounds treated with FGF2, a PCR network revealed the regulation of EMT related genes, including the signaling of the transformation growth factor (TGF). In addition, FGF2 treated wound keratinocytes have expressed transcription factors associated with EMT, including SNAI2, and showed the translocation of β-catenin of the cytoplasm / core cell membrane. However, the in vitro examination of keratinocytes revealed that the FGF2 did not activate EMT in keratinocytes, but that FGF2 could promote EMT in combination with TGFβ1. These results suggest that the treatment of FGF2 wounds could promote Kératinocyte EMT, accelerate the closure of the wound.
Effect of HBO therapy on adipose-derived stem cells, fibroblasts and co-cultures: In vitro study of oxidative stress, angiogenic potential and production of pro-inflammatory growth factors in co-cultures1
Restricted time power enhances the reproduction function of female mice via the liver growth factor 21
Background: There has been a significant increase in epidemic levels, obese women and overweight reproductive age, causing deficiencies to reproductive health. Limited diet (TRF), including isocaltic consumption, has been preventive disorders related to obesity. However, its therapeutic capacity to improve the women’s reproduction function remains largely unknown.
Methods: Here, we investigated the CFR capacity to improve the reproduction function in wild and liver Knockout FGF21 female mice. To study fertility, a continuous and short-term fertility test, a hormone release of gonadotropin (GNRH) and a kisspeptine test were performed. The immortalized GNRH neuron was used to examine the direct role of the liver-liver growth factor (FGF21) on the secretion of GNRH.
Results: We found that Trf saves that the feminine mouse of body weight gain and glucose intolerance, as well as the loss of ovarian follicle and the dysfunction of cyclic prints induced by high fat. In addition, the beneficial effects of the criminal performance of the reproduction performance were also observed in the mouse powered by both Chow and fat. However, these beneficial effects of the Trf on metabolism and reproduction were absent in the Knockout mice of FGF21 specific liver. In vitro, FGF21 directly acted on GNRH neurons to modulate GNRH secretion via an extracellular regulating protein (ERK1 / 2).
Conclusions: Overall, the temporary power supply improves the reproduction function of female mice and the liver’s FGF21 signaling plays a key role in the NEURON GNRH activity in female mice.
Description: Quantitativesandwich ELISA kit for measuring Bovine fibroblast growth factor 21 (FGF21) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Bovine fibroblast growth factor 21 (FGF21) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Human Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human fibroblast growth factor-21,FGF-21 ELISA Kit
Description: Recombinant Fibroblast Growth Factor 21 is a disulfide-linked monomer protein consisting of 182 amino acid residues, and migrates as an approximately 20 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Fibroblast Growth Factor-21 mature chain was expressed in E. coli.
Description: Recombinant Fibroblast Growth Factor 21 is a disulfide-linked monomer protein consisting of 182 amino acid residues, and migrates as an approximately 20 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Fibroblast Growth Factor-21 mature chain was expressed in E. coli.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Human Fibroblast Growth Factor 21 (FGF21) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Fibroblast Growth Factor 21 (FGF21) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Mouse Fibroblast growth factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Fibroblast growth factor 21(FGF21) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rat fibroblast growth factor 21 (FGF21) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat fibroblast growth factor 21 (FGF21) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Guinea pig Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Fibroblast Growth Factor 21 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human FGF21/ Fibroblast growth factor 21 ELISA Kit
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Fibroblast growth factor 21 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Fibroblast Growth Factor 21 (FGF21) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Fibroblast Growth Factor 21 (FGF21) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Fibroblast Growth Factor 21 (FGF21) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Fibroblast Growth Factor 21 (FGF21) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Fibroblast Growth Factor 21 (FGF21) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Fibroblast Growth Factor 21 (FGF21) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Recombinant Rat Fibroblast Growth Factor 21 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells
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Alcoholic cardiomyopathy (CMA) resulting from chronic alcohol abuse is one of the main contributors leading to heart failure and cardiovascular mortality. The growth factor for fibroblasts 21 (FGF21) is a well-established cardioprotective factor. We were aimed at studying the role of FGF21 in experimentally induced models and clinical patients affected by cardiac damage due to chronic alcohol consumption. We have found that the flow of FGF21 levels and FGF21 and β-klotho high protein levels have been increased in subjects with chronic alcohol consumption.
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